DBSCR - Regulated genes

   Database of transcriptional regulation in Streptomyces coelicolor
Home
 Transcription factors
 Regulated genes
 Revised TLS
 Search
 Contacts

Regulated gene: pikAI

Operons: pikAI-pikAV

Genes Gene ID Synonyms Function
pikAI
pikAII
pikAIII
pikAIV
pikAV

Reference: Wilson DJ, et al. (2001)

Promoters

Binding
factor		 Promoter
name		 Regulation Location Binding seq.(cis-element) Experimental evidence Organism
PikD - Positive ND ND Jung WS, et al. (2008): RT-PCR, OV
Wilson DJ, et al. (2001): DB, RG (Direct/Indirect is not clear)
 S. venezuelae
AfsR-sv - Positive ND ND Maharjan S, et al. (2009): OV, RT-PCR
Maharjan S, et al. (2008): -
 S. venezuelae

Comments:
PikD A genetic analysis of PikD using the xylE reporter system showed that the PikD regulator activates the pikAI promoter, whereas it directly or indirectly represses desI promoter, and does not participate in pikC regulation in wild-type S. venezuelae. Gene expression analysis by reverse transcriptase PCR and bioconversion experiments of 10-deoxymethynolide, narbonolide, and TL into the corresponding desosaminyl macrolides indicated that PikD is a positive regulator of the des and pikC genes, as well as the Pik PKS genes.
AfsR-sv RT-PCR analysis was carried out to assess the expression levels of two representative genes: the ketosynthase (KS) gene and pikD. RT-PCR assays demonstrated that afsR-sv functions as a positive regulator and is involved in the regulation of pikromycin biosynthesis in S. venezuelae. Overexpression of afsR-sv in S. venezuelae seemed to activate the transcription of ketosynthase and a pathway-specific regulator, both resulting in the overproduction of pikromycin.

Terminator

No Data

DBSCR:

Copyright: BASE, RIKEN
Contact: Yuko Makita