DBSCR - Transcription factor

   Database of transcriptional regulation in Streptomyces

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Transcription factor: HrdB

GenBank ID no data
Factor type ND
Pfam None
Consensus seq. ND
Comment A mutant library of hrdB gene was constructed by error-prone PCR and selected by high-throughput screening. As a result of evolved hrdB expressed in the modified avermectin high-producing strain, 6.38 g?L of avermectin B1a was produced with over 50% yield improvement, in which the transcription level of aveR was significantly increased. The relevant residues were identified to center in the conserved regions.

Regulated Gene Sigma Regulation Location Binding seq.(cis-element) Experimental evidence Organism
aveR None Promoter -50:+10 ATCAAAGTCAGCGACCTTGACACTACCCCGCCCACCAGGGCAAATTCATAACACTGACCA Zhuo Y, et al. (2010): HM, RO, SDM
S. avermilitis
rrnD1 None Promoter -50:+10 CGCCCCGCAGGAGCCGTTGACACGGCTCAGGCGAGGAGGTAGATTTGAACAGTTGCCTAG Zhuo Y, et al. (2010): HM, RO, SDM
S. avermilitis




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Contact: Yuko Makita