DBSCR - Regulated genes

   Database of transcriptional regulation in Streptomyces coelicolor
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Regulated gene: sigE

Operons: sigE sigE-cseABC

Genes Gene ID Synonyms Direction Genome position Function
sigE SCO3356 SCE94.07, sig28, sigE + 3715604..3716137 ECF sigma factor
sigE SCO3356 SCE94.07, sig28, sigE + 3715604..3716137 ECF sigma factor
cseA SCO3357 SCE94.08, cseA + 3716194..3716802 hypothetical protein
cseB SCO3358 SCE94.09, cseB + 3716926..3717630 two-component system response regulator
cseC SCO3359 SCE94.10, cseC + 3717647..3719170 putative sensory histidine kinase. Contains possible hydrophobic membrane spanning regions

Operon evidence: S1 nuclease mapping: readthrough to cseABC are also weakly confirmed sigE and cseABC are encoded in an operon in which >90% transcription from the sigE ptomoter terminates just downstream of sigE.
Reference: Paget MS, et al. (1999), Paget MS, et al. (1999), Hutchings MI, et al. (2006)

Promoters

Binding
factor		 Promoter
name		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
Unknown_sigma - Promoter -50:+10 3715463..3715522 CCGCAGGCTGCATCGCACAGCGCCGTGACAACGCTTCCCTACCTTCGTTTGTGTGCCCCC Paget MS, et al. (1999): S1, DB
CseB - Positive ND ND ND Paget MS, et al. (1999): S1, DB

Comments:
Unknown_sigma SigE promoter sequence is similar to whiG promoter. Paget MS et al. (1999) also showed that sigE is negatively autoretulated.
CseB sigE transcript was completely absent in cseB mutant; the sigE promoter is absolutely dependent on cseB.

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
GGGACCCGGAGAACCACGCAGGCGGCGAGTACGGCGGTGGCCGTGTTCGTCGCCCTCGGCGTTTCCCTGGCCG
                    >>>>>>>>>>>>>       <<<<<<<<<<<<<<<
3716178..3716212
41..75
-
sigE

Overview

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Contact: Yuko Makita