The purified rAreB protein protects a sequence extending from positions -39 to -26 with respect to the transcriptional start point of areB gene. The protected
region CAAGTTCAAAGTTC overlaps with the putative -35 region (AACTTT) for areB, and it is located 78 nt upstream of the calculated start point for leuC. AreB plays a positive role on leuC and brp transcription.