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Promoters
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| AfsR-sv |
RT-PCR analysis was carried out to assess the expression levels of two representative genes: the ketosynthase (KS) gene and pikD. RT-PCR assays demonstrated that afsR-sv functions as a positive regulator and is involved in the regulation of pikromycin biosynthesis in S. venezuelae. Overexpression of afsR-sv in S. venezuelae seemed to activate the transcription of ketosynthase and a pathway-specific regulator, both resulting in the overproduction of pikromycin. |
Terminator
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