DBSCR - Regulated genes

   Database of transcriptional regulation in Streptomyces coelicolor
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Regulated gene: srrZ

Operon: No data
Genes Gene ID Synonyms Function
srrZ

Promoters

Binding
factor		 Promoter
name		 Regulation Location Binding seq.(cis-element) Experimental evidence Organism
Unknown_sigma - Promoter -50:+10 ACGGCGGTGGAGATACCGGAGCCGGACGGGAGAACGCGGCTACGGTGCAGACGCGCCGGTG Suzuki T, et al. (2010): HM, S1
 S. rochei
SrrY - Positive -108:-24 GCGGTGGTTCGCCGGACCGGGCGGGGGAGCGCCGCTTCCGCTTGGAGTGGGGCTCGAACGGCGGTGGAGATACCGGAGCCGGAC Suzuki T, et al. (2010): HM, S1, DB, DP, OV, GS
 S. rochei

Comments:
Unknown_sigma We determined the transcriptional start site (TSS) of srrZ by high-resolution S1 nuclease protection assay, and identified it 52bp upstream of translational start coden of srrZ. Immediately upstream of the TSS, apossible -10 sequence (TACGGT) was identified. It was similar to the consensus -10 sequence (TAGRRT, R = A or G) of streptomycete Esigma70-like promoters. However, the -35 sequence (consensus TTGACR) was not found at the corresponding of the TSS of srrZ.
SrrY The SrrY protein was specifically bound to the promoter region of srrZ, where a possible SARP binding sequence was identified 26 bp upstream of the -10 sequence. Deletion of the repeat sequences from this region abolished its SrrY binding activity. In addition, complementation of srrZ restored lankamycin production in the srrY mutant. All of these results confirm that the SARP gene srrY directly regulates expression of the second SARP gene srrZ in a positive manner.

Terminator

No Data

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Contact: Yuko Makita