DBSCR - Regulated genes

   Database of transcriptional regulation in Streptomyces coelicolor

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Regulated gene: sanN

Operons: sanNMLKJHI

Genes Gene ID Synonyms Function
sanN
sanM
sanL
sanK
sanJ
sanH
sanI

Reference: Ling HB, et al. (2008)


Promoters

Binding
factor
Promoter
name
Regulation Location Binding seq.(cis-element) Experimental evidence Organism
Unknown_sigma sanN-p1? Promoter -50:+10 GTCAGTGCACGGCAAGCCGTCGGCAAGGGCCCCAGCTCCAGGCTGGGCCGCGTAGGGACCA Liu G, et al. (2005): DB
He X, et al. (2010): GS, FT, SDM
Ling HB, et al. (2008): PE
S. ansochromogenes
SanG sanN-p1? Positive -54:-23 GCCGGTCAGTGCACGGCAAGCCGTCGGCAAGG Liu G, et al. (2005): DB
He X, et al. (2010): GS, FT, SDM
Ling HB, et al. (2008): PE
S. ansochromogenes
SanG sanN-p1? Positive -54:-23 GCCGGTCAGTGCACGGCAAGCCGTCGGCAAGG Liu G, et al. (2005): DB
He X, et al. (2010): GS, FT, SDM
Ling HB, et al. (2008): PE
S. ansochromogenes

Comments:
Unknown_sigma A DNase I footprinting assay was used to analyse the interaction of SanG with the bidirectional sanN-sanO promoter region. On the antisense strand, two regions stretching from positions -7 to -47 and from -58 to -89 bases relative to the transcription start point of sanO were protected by SanG. Analysis of the SanG-binding region revealed two direct repeats 5'-CGGCAAG-3' that overlap with the -35 regions of the sanN promoter P1 and sanO promoter, respectively. This is a typical feature of SARP-binding targets.
SanG A DNase I footprinting assay was used to analyse the interaction of SanG with the bidirectional sanN-sanO promoter region. On the antisense strand, two regions stretching from positions -7 to -47 and from -58 to -89 bases relative to the transcription start point of sanO were protected by SanG. Analysis of the SanG-binding region revealed two direct repeats 5'-CGGCAAG-3' that overlap with the -35 regions of the sanN promoter P1 and sanO promoter, respectively. This is a typical feature of SARP-binding targets.
SanG A DNase I footprinting assay was used to analyse the interaction of SanG with the bidirectional sanN-sanO promoter region. On the antisense strand, two regions stretching from positions -7 to -47 and from -58 to -89 bases relative to the transcription start point of sanO were protected by SanG. Analysis of the SanG-binding region revealed two direct repeats 5'-CGGCAAG-3' that overlap with the -35 regions of the sanN promoter P1 and sanO promoter, respectively. This is a typical feature of SARP-binding targets.


Terminator

No Data




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Contact: Yuko Makita