DBSCR - Regulated genes

   Database of transcriptional regulation in Streptomyces coelicolor
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Regulated gene: redD

Operon: No data
Genes Gene ID Synonyms Direction Genome position Function
redD SCO5877
+ 6432566..6433618 transcriptional regulator RedD

Promoters

Binding
factor		 Promoter
name		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
HrdD redD-p Promoter -50:+10 6432548..6432607 AATCCGATCGTTCGGTGGATGACGGGTGGGGGAGTGCTTGCCACGATGGACCCGGTTCGA Fujii T, et al. (1996): RO
Kang JG, et al. (1997): GS, RO
RedZ - Positive ND ND ND White J, et al. (1997): S1, DB
AfsR - Positive ND ND ND Floriano B, et al. (1996): OV (direct/indirect regulation is not clear), DB showed no effect.
HrdB redD-p Promoter -50:+10 6432548..6432607 AATCCGATCGTTCGGTGGATGACGGGTGGGGGAGTGCTTGCCACGATGGACCCGGTTCGA Fujii T, et al. (1996): DB, SDS-PAGE
Aigle B, et al. (2000): SDM
BldC - Positive ND ND ND Hunt AC, et al. (2005): DB,OV
WblA - Negative ND ND ND Kang SH, et al. (2007): AR, OV(RT-PCR)
RrdA - Negative ND ND ND Ou X, et al. (2009): DB,OV
SCO1712 - Negative ND ND ND Lee HN, et al. (2010): DB, OV(by RT-PCR)
Kang SH, et al. (2007): AR
SCO6008 - ND ND ND ND Park SS, et al. (2009): DACA(DNA-affinity capture assay; mass spectrometry), GS, DB
AdpA - Negative? ND ND ND Park SS, et al. (2009): DACA(DNA-affinity capture assay; mass spectrometry), GS, DB
SCO5405 - ND ND ND ND Park SS, et al. (2009): DACA(DNA-affinity capture assay; mass spectrometry), GS, DB
SCO1480 - ND ND ND ND Park SS, et al. (2009): DACA(DNA-affinity capture assay; mass spectrometry), GS, DB

Comments:
HrdD RedD must be regulated by at leaset one other sigma factor because disruption of hrdD had no effect on the production of redD. Fujii et al. surmise hrdB as a candidate. Kim YJ, et al. 2008(PMID: 19087294) also suggested sigH is also strongly upregulated by the pH shock.
RedZ Transcription of redD strongly dependes on redZ. Although redZ does not depend on bldA (tRNA for rare leucine codon UUA) mutant, redD transcription was undetectable in bldA mutant (White J, et al. JB 1997, PMID: 9006013).
SCO1712 Transcripts encoded by pathway-specific activator genes such as actII-ORF4, redD/Z, and cdaR were all significantly increased at both time points from S. coelicolor M145-SCO1712. As expected, an opposite transcription pattern was observed in the SCO1712-overexpressing S. coelicolor M145 strain.
SCO6008 SCO6008 seemed to discriminate the two promoter regions, binding to only one of the two promoter regions, redD. our EMSA experiments showed the binding of SCO6008 onto the promoter of redD, not actII-ORF4, and that the direct and/or indirect regulation of SCO6008 on Act biosynthesis was quite possible.
AdpA In S. coelicolor, the adpA-deletion mutant was found to be unable to produce Act, but to overproduce Red. AdpA, SCO5405, and SCO1480 showed binding activities to both the actII-ORF4 (PactII-ORF4) and redD (PredD) promoter regions.
SCO5405 AdpA, SCO5405, and SCO1480 showed binding activities to both the actII-ORF4 (PactII-ORF4) and redD (PredD) promoter regions.
SCO1480 AdpA, SCO5405, and SCO1480 showed binding activities to both the actII-ORF4 (PactII-ORF4) and redD (PredD) promoter regions. However, the binding of SCO1480 to PactII-ORF4 was not detected in DACA.

Terminator

No Data

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Contact: Yuko Makita