DBSCR - Regulated genes

   Database of transcriptional regulation in Streptomyces coelicolor
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Regulated gene: actII-ORF4

Operon: No data
Genes Gene ID Synonyms Direction Genome position Function
actII-ORF4 SCO5085 SCBAC28G1.11, actII-4, actII-ORF4 + 5528094..5528861 actinorhodin cluster activator protein

Promoters

Binding
factor		 Promoter
name		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
HrdD actII-ORF4p Promoter -50:+10 5528013..5528072 TGACGGCAAGCACATTGAAATCTGTTGAGTAGGCCTGTTATTGTCGCCCCCAGGAGACGG Fujii T, et al. (1996): RO
Kang JG, et al. (1997): GS, RO
AbsA2 - Negative ND ND ND McKenzie NL, et al. (2007): ChIP, DB, GS
Aceti DJ, et al. (1998): DB
AmfC - Positive ND ND ND Yonekawa Y, et al. (1999): DB
AfsR - Positive ND ND ND Floriano B, et al. (1996): OV (direct/indirect regulation is not clear), DB showed no effect.
HrdB actII-ORF4p Promoter -50:+10 5528013..5528072 TGACGGCAAGCACATTGAAATCTGTTGAGTAGGCCTGTTATTGTCGCCCCCAGGAGACGG Fujii T, et al. (1996): DB, PAGE
Aigle B, et al. (2000): SDM
AtrA - Positive ND 5527887..5527915 CGTATCAGGAATGCCAGATTCTATTGATT Uguru GC, et al. (2005): GS, FT, DP, DB
AtrA - Positive ND 5528131..5528166 CCGATGCGGGATGTGTAATTCCGCTTAAATCCTCGA Uguru GC, et al. (2005): GS, FT, DP, DB
BldC - Positive ND ND ND Hunt AC, et al. (2005): DB,OV
NsdA - Negative ND ND ND Li W, et al. (2006): DB, HB
WblA - Negative ND ND ND Kang SH, et al. (2007): AR, OV(RT-PCR)
RapA1 - Positive ND ND ND Li Y, et al. (2007): DB (RT-PCR)
RapA1 - Positive ND ND ND Li Y, et al. (2007): DB (RT-PCR)
DasR - Negative -71:-31(from translation start site) 5528023..5528062 CACATTGAAATCTGTTGAGTAGGCCTGTTATTGTCGCCCC Rigali S, et al. (2008): HM, GS, sqRT-PCR
SCO1712 - Negative ND ND ND Lee HN, et al. (2010): DB, OV(by RT-PCR)
Kang SH, et al. (2007): AR
AdpA - Positive? ND ND ND Park SS, et al. (2009): DACA(DNA-affinity capture assay; mass spectrometry), GS, DB
SCO5405 - ND ND ND ND Park SS, et al. (2009): DACA(DNA-affinity capture assay; mass spectrometry), GS, DB
SCO1480 - ND ND ND ND Park SS, et al. (2009): DACA(DNA-affinity capture assay; mass spectrometry), GS, DB

Comments:
HrdD ActII-ORF4 must be regulated by at leaset one other sigma factor because disruption of hrdD had no effect on the production of actII-ORF4. Fujii et al. surmise hrdB as a candidate.
DasR The dre site upstream from redZ is situated about 50 bp upstream from the -35 sequence of the redZ promoter. DasR bound to the dre elements for the actII-ORF4, redZ and crr-ptsI promoter regions, but not to the cis-acting element of Crp. Semiquantitative reverse transcription-PCR (RT-PCR) analyses further showed upregulation of actII-ORF4 and redZ in the dasR mutant.
SCO1712 Transcripts encoded by pathway-specific activator genes such as actII-ORF4, redD/Z, and cdaR were all significantly increased at both time points from S. coelicolor M145-SCO1712. As expected, an opposite transcription pattern was observed in the SCO1712-overexpressing S. coelicolor M145 strain.
AdpA In S. coelicolor, the adpA-deletion mutant was found to be unable to produce Act, but to overproduce Red. AdpA, SCO5405, and SCO1480 showed binding activities to both the actII-ORF4 (PactII-ORF4) and redD (PredD) promoter regions.
SCO5405 AdpA, SCO5405, and SCO1480 showed binding activities to both the actII-ORF4 (PactII-ORF4) and redD (PredD) promoter regions.
SCO1480 AdpA, SCO5405, and SCO1480 showed binding activities to both the actII-ORF4 (PactII-ORF4) and redD (PredD) promoter regions. However, the binding of SCO1480 to PactII-ORF4 was not detected in DACA.

Terminator

No Data

Overview

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Contact: Yuko Makita